Difference between revisions of "Part:BBa K4129002"

Revision as of 15:13, 9 October 2022

Promoter of the Aspergillus niger FDH gene

The part consists of the 1000 bp upstream of Formate Dehydrogenase (FDH) CDS from Aspergillus niger. Since FDH genes have been shown to be upregulated in response to furfural in Saccharomyces cerevisiae, the part was utilised to test if anFDH promoter is also responsive to furfural. When used to drive expression of mCherry in A. niger through transient expression, it is neither constitutive nor induced by furfural or does not promote expression at levels high enough to be detected during plasmid based expression.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 34
Illegal SpeI site found at 160
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 34
Illegal SpeI site found at 160
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 34
Illegal SpeI site found at 160
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 34
Illegal SpeI site found at 160
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 564
Illegal BsaI site found at 929

Revision as of 13:06, 2 October 2022 (view source) GCG (Talk \| contribs) ← Older edit		Revision as of 15:13, 9 October 2022 (view source) GCG (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K4129002 short</partinfo>		<partinfo>BBa_K4129002 short</partinfo>

−	The part consists of the 1000 bp upstream of Formate Dehydrogenase (FDH) CDS from Aspergillus niger. Since FDH genes have been shown to be upregulated in response to furfural in Saccharomyces cerevisiae, the part was utilised to test if anFDH promoter is also responsive to furfural. When used to drive expression of mCherry in A. niger through transient expression, ~~the promoter does not appear to be~~ induced by furfural or does not promote expression at levels high enough to be detected during ~~transient~~ expression.	+	The part consists of the 1000 bp upstream of Formate Dehydrogenase (FDH) CDS from Aspergillus niger. Since FDH genes have been shown to be upregulated in response to furfural in Saccharomyces cerevisiae, the part was utilised to test if anFDH promoter is also responsive to furfural. When used to drive expression of mCherry in A. niger through transient expression, it is neither constitutive nor induced by furfural or does not promote expression at levels high enough to be detected during plasmid based expression.