Difference between revisions of "Part:BBa K4129000"

Latest revision as of 15:11, 9 October 2022

Promoter of the Saccharomyces cerevisiae FDH1 gene

The part consists of the 1000 bp upstream of Formate Dehydrogenase 1 (FDH1) CDS from Saccharomyces cerevisiae. FDH1 has been shown to be upregulated in response to furfural by transcriptomics data. The part was amplified the part from gDNA through PCR and used it to test if the promoter responded to furfural in Aspergillus niger as well. When used to drive expression of mCherry in A. niger through transient expression, it is neither constitutive nor induced by furfural or does not promote expression at levels high enough to be detected during plasmid based expression.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 278
Illegal XhoI site found at 519
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 193
Illegal BsaI.rc site found at 345

Revision as of 13:03, 2 October 2022 (view source) GCG (Talk \| contribs) ← Older edit		Latest revision as of 15:11, 9 October 2022 (view source) GCG (Talk \| contribs)
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−	The part consists of the 1000 bp upstream of Formate Dehydrogenase 1 (FDH1) CDS from Saccharomyces cerevisiae. FDH1 has been shown to be upregulated in response to furfural by transcriptomics data. The part was amplified the part from gDNA through PCR and used it to test if the promoter responded to furfural in Aspergillus niger as well. When used to drive expression of mCherry in A. niger through transient expression, it is ~~not active in A. niger~~ or does not promote expression at levels high enough to be detected during plasmid based expression.	+	The part consists of the 1000 bp upstream of Formate Dehydrogenase 1 (FDH1) CDS from Saccharomyces cerevisiae. FDH1 has been shown to be upregulated in response to furfural by transcriptomics data. The part was amplified the part from gDNA through PCR and used it to test if the promoter responded to furfural in Aspergillus niger as well. When used to drive expression of mCherry in A. niger through transient expression, it is neither constitutive nor induced by furfural or does not promote expression at levels high enough to be detected during plasmid based expression.