Difference between revisions of "Part:BBa K4227021"

 
 
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<partinfo>BBa_K4227021 short</partinfo>
 
<partinfo>BBa_K4227021 short</partinfo>
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<p>P<sub>xyl</sub> is a kind of promotor that can be started by the xylose.</p>
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a
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<p>tetR-ssrA is an improved version of tetracycline resistance transcriptional repressor (tetR), which is registered by 10_Heidelberg iGEM team (BBa_K337028: tet Repressor). </p>
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<p>SsrA is a degradation tag, added at the 3' end of tetR that degrades the repressor protein tetR to become tetR-ssrA, thus we endow the original part a new function as improvement.</p>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 07:22, 12 October 2022


Pxyl-TetR-ssrA

Pxyl is a kind of promotor that can be started by the xylose.

tetR-ssrA is an improved version of tetracycline resistance transcriptional repressor (tetR), which is registered by 10_Heidelberg iGEM team (BBa_K337028: tet Repressor).

SsrA is a degradation tag, added at the 3' end of tetR that degrades the repressor protein tetR to become tetR-ssrA, thus we endow the original part a new function as improvement.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 92
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 734
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 92
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 92
  • 1000
    COMPATIBLE WITH RFC[1000]