Difference between revisions of "Part:BBa K4180003"

Line 13: Line 13:
 
[[File:      ??put picture link in here??      ]]
 
[[File:      ??put picture link in here??      ]]
 
[[File:      ??put picture link in here??      |200px|thumb|left|    ??you can write the command in this part??    ]]-->
 
[[File:      ??put picture link in here??      |200px|thumb|left|    ??you can write the command in this part??    ]]-->
[[File:BBa K4180002.png|400px|thumb|left|<h3>&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;BBa K4180002 <br>&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;snf1Δ2-306aa
+
[[File:BBa K4180003.png|400px|thumb|left|<h3>&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;BBa K4180003 snf1Δ381-633aa<br>&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;PCR product on lane 3 compared to<br>&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;BBa K4180002 snf1Δ2-306aa
<br>&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp; PCR product </h3>]]
+
<br>&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp; PCR product on lanes 1 and 2 </h3>]]
 
[[File:BBa K4180002-2.png|400px|thumb|left|<h3>After Bacteria transformation, bacteria colonies were picking up directly to do snf1Δ2-306aa - colonies had BBa_K4180002 snf1Δ2-306aa<br> &emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp; PCR product </h3>]]
 
[[File:BBa K4180002-2.png|400px|thumb|left|<h3>After Bacteria transformation, bacteria colonies were picking up directly to do snf1Δ2-306aa - colonies had BBa_K4180002 snf1Δ2-306aa<br> &emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp; PCR product </h3>]]
  

Revision as of 08:50, 2 October 2022


snf1Δ381-633aa C-truncated

SNF1Δ381-633aa, C-terminus truncated from amino acid381 to 633 deleting autoinhibitory domain and SIP-interacting domain (SIR) Sequence and Features

        BBa K4180003 snf1Δ381-633aa
        PCR product on lane 3 compared to
        BBa K4180002 snf1Δ2-306aa
         PCR product on lanes 1 and 2

After Bacteria transformation, bacteria colonies were picking up directly to do snf1Δ2-306aa - colonies had BBa_K4180002 snf1Δ2-306aa
         PCR product



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 528
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]