Difference between revisions of "Part:BBa K4180000"
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[[File:BBa K4180000.png|400px|thumb|left|<h3>     BBa_K4180000 SNF1 full length <br>          PCR product </h3>]] | [[File:BBa K4180000.png|400px|thumb|left|<h3>     BBa_K4180000 SNF1 full length <br>          PCR product </h3>]] | ||
| − | [[File:BBa K4180000-2.png|400px|thumb|left|<h3> | + | [[File:BBa K4180000-2.png|400px|thumb|left|<h3>After Bacteria transformation, bacteria colonies were picking up directly to do SNF1 full length PCR - colonies #1, 3, 5, and 6 had BBa_K4180000(SNF1 full length)<br>          PCR product </h3>]] |
Revision as of 08:22, 2 October 2022
SNF1 is the metabolism sensor
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AMP is an energy sensor regulated by energy depletion, such as AMP (adenosine monophosphate): ATP (adenosine triphosphate) ration is high, AMPK is induced by upstream pathway to act as the master regulator of energy metabolism, it will increase catabolic process, such as triggering fatty acid catabolism for more ATP, and reduce anabolic process, such as protein and fatty acid synthesis to reduce energy consumed (Herzig and Shaw 2018)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 528
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]