Difference between revisions of "Part:BBa K4380011:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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The design of this part used exploited type IIs restriction enzymes LguI ability to recognize asymmetric DNA sequences and cleave at a defined distance outside of its recognition sequence.
+
The design of this part used exploited type IIs restriction enzymes LguI ability to recognize asymmetric DNA sequences and cleave at a defined distance outside of its recognition sequence. <b>
 +
When optimizing a vector sequence to use with this specific part, optimizations need to be perfomed so that LguI does not cleave DNA in any other spot, other than the plasmid and its insert.
  
 
===Source===
 
===Source===

Revision as of 21:19, 3 October 2022


eGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 151
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 16


Design Notes

The design of this part used exploited type IIs restriction enzymes LguI ability to recognize asymmetric DNA sequences and cleave at a defined distance outside of its recognition sequence. When optimizing a vector sequence to use with this specific part, optimizations need to be perfomed so that LguI does not cleave DNA in any other spot, other than the plasmid and its insert.

Source

Synthetic

===References===