Difference between revisions of "Part:BBa K4201009"

 
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{|width='80%'
 
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[[Image:Terminator.png]]
 
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<partinfo>BBa_K4201009 parameters</partinfo>
 
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<partinfo>BBa_K4201009 short</partinfo>
 
<partinfo>BBa_K4201009 short</partinfo>
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* Description
 
  
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AtHSP is a terminator that was discovered from the analysis of the A. thaliana genome<sup>1</sup>. It contains poly A regions where the mRNA is cleaved, which helps increase the expression of the desired transgene<sup>1</sup>.  When compared to other A. thaliana terminators, AtHSP was effective in increasing expression levels of the desired transgene<sup>1,2</sup>.
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Heat shock proteins (HSPs) are involved in many different essential cellular processes, which makes AtHSP a good candidate for increasing the expression of a transgene<sup>3</sup>.  Additional benefits of using AtHSP include its small size, which helps reduce the overall size of the desired genetic construct.
  
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'''Secondary Structure'''
 
  
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[[Image:Mfold-K4201009-1.png]]
 
  
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<hr>
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<!-- Add more about the biology of this part here
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'''Measurement'''
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===Usage and Biology===
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* [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4201007 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K4201007 parameters</partinfo>
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Revision as of 02:15, 12 October 2022


Arabidopsis thaliana Heat Shock Protein (AtHSP)

AtHSP is a terminator that was discovered from the analysis of the A. thaliana genome1. It contains poly A regions where the mRNA is cleaved, which helps increase the expression of the desired transgene1. When compared to other A. thaliana terminators, AtHSP was effective in increasing expression levels of the desired transgene1,2. Heat shock proteins (HSPs) are involved in many different essential cellular processes, which makes AtHSP a good candidate for increasing the expression of a transgene3. Additional benefits of using AtHSP include its small size, which helps reduce the overall size of the desired genetic construct.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 184
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 184
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 184
    Illegal BglII site found at 800
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 184
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 184
    Illegal NgoMIV site found at 333
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1517