Difference between revisions of "Part:BBa K4129000:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Since we do not know what motifs are responsible for the promoter being upregulated by furfural in S. cerevisiae, we did not modify the sequence at all (even leaving BsaI sites)
+
The part was designed to be used with USER cloning. BsaI sites were not removed to avoid accidentally affecting the promoter activity.
−
 
+
  
  
 
===Source===
 
===Source===
  
−
The part was obtained from gDNA
+
The part was obtained from gDNA.
  
 
===References===
 
===References===

Latest revision as of 09:52, 2 October 2022


Promoter of the Saccharomyces cerevisiae FDH1 gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 278
    Illegal XhoI site found at 519
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 193
    Illegal BsaI.rc site found at 345


Design Notes

The part was designed to be used with USER cloning. BsaI sites were not removed to avoid accidentally affecting the promoter activity.


Source

The part was obtained from gDNA.

References