Difference between revisions of "Part:BBa K4324100:Design"
(→‎Source)
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This original protein sequence was taken from the XYL1 gene of Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545), which can be found on the [https://rest.uniprot.org/uniprotkb/P31867.fasta Uniprot database].
 
This original protein sequence was taken from the XYL1 gene of Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545), which can be found on the [https://rest.uniprot.org/uniprotkb/P31867.fasta Uniprot database].
  
 +
Alternatively, the protein sequence is pasted below:
 +
<syntaxhighlight>
 
MPSIKLNSGYDMPAVGFGCWKVDVDTCSEQIYRAIKTGYRLFDGAEDYANEKLVGAGVKK
 
MPSIKLNSGYDMPAVGFGCWKVDVDTCSEQIYRAIKTGYRLFDGAEDYANEKLVGAGVKK
 
AIDEGIVKREDLFLTSKLWNNYHHPDNVEKALNRTLSDLQVDYVDLFLIHFPVTFKFVPL
 
AIDEGIVKREDLFLTSKLWNNYHHPDNVEKALNRTLSDLQVDYVDLFLIHFPVTFKFVPL
Line 19: Line 21:
 
IKAIAAKHGKSPAQVLLRWSSQRGIAIIPKSNTVPRLLENKDVNSFDLDEQDFADIAKLD
 
IKAIAAKHGKSPAQVLLRWSSQRGIAIIPKSNTVPRLLENKDVNSFDLDEQDFADIAKLD
 
INLRFNDPWDWDKIPIFV
 
INLRFNDPWDWDKIPIFV
 +
</syntaxhighlight>
  
 
===References===
 
===References===
 
1. https://www.uniprot.org/uniprotkb/P31867/entry
 
1. https://www.uniprot.org/uniprotkb/P31867/entry
 
2. https://www.genome.jp/dbget-bin/www_bget?ec:1.1.1.307
 
2. https://www.genome.jp/dbget-bin/www_bget?ec:1.1.1.307

Revision as of 14:21, 1 October 2022


NAD(P)H-dependent D-xylose reductase from S. stipitis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 747


Design Notes

The protein sequence was codon-optimised for Escherichia coli through the IDT codon optimisation tool as gBlocks Gene Fragments.

Source

This original protein sequence was taken from the XYL1 gene of Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545), which can be found on the Uniprot database.

Alternatively, the protein sequence is pasted below: <syntaxhighlight> MPSIKLNSGYDMPAVGFGCWKVDVDTCSEQIYRAIKTGYRLFDGAEDYANEKLVGAGVKK AIDEGIVKREDLFLTSKLWNNYHHPDNVEKALNRTLSDLQVDYVDLFLIHFPVTFKFVPL EEKYPPGFYCGKGDNFDYEDVPILETWKALEKLVKAGKIRSIGVSNFPGALLLDLLRGAT IKPSVLQVEHHPYLQQPRLIEFAQSRGIAVTAYSSFGPQSFVELNQGRALNTSPLFENET IKAIAAKHGKSPAQVLLRWSSQRGIAIIPKSNTVPRLLENKDVNSFDLDEQDFADIAKLD INLRFNDPWDWDKIPIFV </syntaxhighlight>

References

1. https://www.uniprot.org/uniprotkb/P31867/entry 2. https://www.genome.jp/dbget-bin/www_bget?ec:1.1.1.307