Difference between revisions of "Part:BBa K4385011:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | The lead sensor is composed of BBa_K4385009 pbr and BBa_E0040 GFP. Note the order of inducible promoter Pbc, constitutive promoter Pbr and lead-sensing regulatory protein PbrR in pbr. | |
===Source=== | ===Source=== |
Revision as of 14:15, 6 October 2022
Lead sensor
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 462
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 462
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 462
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 462
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 253
Illegal BsaI.rc site found at 1394
Design Notes
The lead sensor is composed of BBa_K4385009 pbr and BBa_E0040 GFP. Note the order of inducible promoter Pbc, constitutive promoter Pbr and lead-sensing regulatory protein PbrR in pbr.
Source
It is amplified from E. coli genomic DNA.