Difference between revisions of "Part:BBa K4488024:Design"

 
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===Design Notes===
 
===Design Notes===
−
Edits have been made to remove restriction sites and optimise for cloning into the plasmid vector for E. coli.  
+
Cytosine at position 153 was changed to thymine and thymine at position 256 was changed to guanine. No restriction sites needed to be removed.
  
  

Revision as of 04:13, 9 October 2022


Modified cellulose binding domain from Clostridium thermocellum cellulosomal scaffold protein A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cytosine at position 153 was changed to thymine and thymine at position 256 was changed to guanine. No restriction sites needed to be removed.


Source

BBa_K1321014

References