Difference between revisions of "Part:BBa K200010"
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This is a Type II restriction enzyme that recognises the sequence TCGA.  Its activity can be blocked by dam methylation. <br>
 
This is a Type II restriction enzyme that recognises the sequence TCGA.  Its activity can be blocked by dam methylation. <br>
  
−
It works at 65 degrees Celsius. <br>
+
The working temperature is 65°C. <br>
  
 
As part of the Imperial 2009 iGEM E.ncapsulator project, TaqI was used along with DpnII to cut up the genetic material within the cell, completing the cell death module.  
 
As part of the Imperial 2009 iGEM E.ncapsulator project, TaqI was used along with DpnII to cut up the genetic material within the cell, completing the cell death module.  

Revision as of 17:23, 2 September 2009

TaqI Composite

This sequence codes for the restriction enzyme TaqI.

This is a Type II restriction enzyme that recognises the sequence TCGA. Its activity can be blocked by dam methylation.

The working temperature is 65°C.

As part of the Imperial 2009 iGEM E.ncapsulator project, TaqI was used along with DpnII to cut up the genetic material within the cell, completing the cell death module.


Usage and Biology

The restriction enzyme TaqI, is isolated from the bacterium Thermus aquaticus. 1

It cuts at a site that is near to where early and late messenger RNA transcription starts in opposite directions. Furthermore, it is a 4 base cutter, leaving a 2 base overhang.

The cutting site is as shown:

TCGA -> T / CGA

AGCT -> AGC / T 1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 36
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 175


References

<biblio>

  1. 1 pmid=204628

</biblio>

2. [http://www.neb.com/nebecomm/products/productM0219.asp Information from NEB]