Difference between revisions of "Part:BBa K4220100"
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K4220100 short</partinfo>
 
<partinfo>BBa_K4220100 short</partinfo>
 +
 +
==Introduction==
  
 
This is a kill switch in yeast which can let the yeast survive only in the presence of copper ions.
 
This is a kill switch in yeast which can let the yeast survive only in the presence of copper ions.
 +
 +
==How to kill the yeast? ==
 +
 +
HSF1: essential gene in yeast [1]
 +
 +
==How to determine when to kill? ==
 +
 +
Copper ion: abundant in the intestine; scarce in the environment [2]
 +
 +
==Others==
 +
 +
HIS3-NatR-ADH1: resistant gene expression for screening
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 12: Line 26:
 
<partinfo>BBa_K4220100 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4220100 SequenceAndFeatures</partinfo>
  
 +
 +
==References==
 +
 +
1 Kopczynski, J. B., Raff, A. C. & Bonner, J. J. Translational readthrough at nonsense mutations in the HSF1 gene of Saccharomyces cerevisiae. Mol Gen Genet 234, 369-378, doi:10.1007/BF00538696 (1992).
 +
 +
2 Nose, Y., Kim, B. E. & Thiele, D. J. Ctr1 drives intestinal copper absorption and is essential for growth, iron metabolism, and neonatal cardiac function. Cell metabolism 4, 235-244, doi:10.1016/j.cmet.2006.08.009 (2006).
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 07:00, 1 October 2022


HSF1up-HIS3+NatR+ADH1+CUP1-HSF ORF

Introduction

This is a kill switch in yeast which can let the yeast survive only in the presence of copper ions.

How to kill the yeast?

HSF1: essential gene in yeast [1]

How to determine when to kill?

Copper ion: abundant in the intestine; scarce in the environment [2]

Others

HIS3-NatR-ADH1: resistant gene expression for screening

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 247
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 367
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 512


References

1 Kopczynski, J. B., Raff, A. C. & Bonner, J. J. Translational readthrough at nonsense mutations in the HSF1 gene of Saccharomyces cerevisiae. Mol Gen Genet 234, 369-378, doi:10.1007/BF00538696 (1992).

2 Nose, Y., Kim, B. E. & Thiele, D. J. Ctr1 drives intestinal copper absorption and is essential for growth, iron metabolism, and neonatal cardiac function. Cell metabolism 4, 235-244, doi:10.1016/j.cmet.2006.08.009 (2006).