Difference between revisions of "Part:BBa K4268000:Experience"
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| − | + | T-suny-oneonta-t7-head-tail connector colony PCR figure.png | |
Caption: Colony PCR of five colonies (clones) suspected of coating the insert Head-tail connector insert. The insert length is 1573bp and the predicted size of the PCR product using VF/VR primers is 1739 bp. | Caption: Colony PCR of five colonies (clones) suspected of coating the insert Head-tail connector insert. The insert length is 1573bp and the predicted size of the PCR product using VF/VR primers is 1739 bp. | ||
Revision as of 22:42, 3 October 2022
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Applications of BBa_K4268000
The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5&alpha cells. After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.
T-suny-oneonta-t7-head-tail connector colony PCR figure.png
Caption: Colony PCR of five colonies (clones) suspected of coating the insert Head-tail connector insert. The insert length is 1573bp and the predicted size of the PCR product using VF/VR primers is 1739 bp.
The gel indicates that all five colonies are likely to contain the correct insert and thus, the Head-Tail Connector was successfully cloned into a Level O Golden Gate Assembly basic part.
User Reviews
UNIQabc503edfa51a9bd-partinfo-00000000-QINU UNIQabc503edfa51a9bd-partinfo-00000001-QINU