Difference between revisions of "Part:BBa K4202010"

Latest revision as of 04:15, 1 October 2022

Amorphous Calcium CarbonateBinding Protein(ACCBP)(without the 16 amino acids at N terminus)

Amorphous calcium carbonate binding protein(ACCBP) is a protein that can help to form and transform amorphous calcium carbonate(ACC).

In calcium carbonate solution，the ions can automatically aggregate and form prenucleation clusters, which further form ACC through certain pathways. ACC will transform into minerals of different crystal forms. In this process, organisms mainly regulate the biological mineralization process by regulating ACC formation and transformation. ACCBP is an important protein that can help to form ACC and transform ACC into calcite at specific conditions.

To ensure that this part can express active protein, the corresponding plasmid should be transformed into the E.coli, and the protein is induced at specific conditions.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 45
Illegal AgeI site found at 308
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 514

@@ Line 7: / Line 7: @@
 Amorphous calcium carbonate binding protein(ACCBP) is a protein that can help to form and transform amorphous calcium carbonate(ACC).
-In calcium carbonate solution&#65292;the ions can automatically aggregate and form prenucleation clusters, which further form ACC through certain pathways. ACC will transform into minerals of different crystal forms. In this process, organisms mainly regulate the biological mineralization process by regulating ACC formation and transformation. ACCBP is an important protein that can help to form ACC and transform ACC into calcite in specific condition.
+In calcium carbonate solution&#65292;the ions can automatically aggregate and form prenucleation clusters, which further form ACC through certain pathways. ACC will transform into minerals of different crystal forms. In this process, organisms mainly regulate the biological mineralization process by regulating ACC formation and transformation. ACCBP is an important protein that can help to form ACC and transform ACC into calcite at specific conditions.
-To ensure that this part can express active protein&#65292;the corresponding plasmid should be transformed into the <i>E.coli</i>, and the protein is induced at specific condition.
+To ensure that this part can express active protein, the corresponding plasmid should be transformed into the <i>E.coli</i>, and the protein is induced at specific conditions.
 <!-- Add more about the biology of this part here