Difference between revisions of "Part:BBa K4165100"

Revision as of 20:14, 6 October 2022

HTRA Binding Peptide 6

part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein

Usage and Biology

Synthetic peptide acquired by Phage display, Binds specifically to PDZ domain of HTRA1 and HTRA3 Proteins with G of –8.0 and IC50 of 3.5 ± 0.9

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Functional Parameters

Got score of 4 out of 6 in our Quality assessment code after modeling by AlphaFold2.0

cbeta_deviations = 0, clashscore = 8.47, molprobity = 2.19, ramachandran_favored = 80, ramachandran_outliers = 0, Qmean_4 = -2.543151, Qmean6 = -1.227686

Modeling

                 Figure 1.: 3D Model of H1F peptide Modelled by AlphaFold2.0 displayed in Pyomol

@@ Line 5: / Line 5: @@
 part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+Synthetic peptide acquired by Phage display, Binds specifically to PDZ domain of HTRA1 and HTRA3 Proteins with G of –8.0 and IC50 of 3.5 ± 0.9
 <!-- -->
@@ Line 15: / Line 12: @@
 <partinfo>BBa_K4165100 SequenceAndFeatures</partinfo>
-<!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
+Got score of 4 out of 6 in our Quality assessment code after modeling by AlphaFold2.0
+cbeta_deviations = 0, clashscore = 8.47, molprobity = 2.19, ramachandran_favored = 80, ramachandran_outliers = 0, Qmean_4 = -2.543151, Qmean6 = -1.227686
+===Modeling===
+<html>
+<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/htra1-bp/h1f.jpg" style="margin-left:200px;" alt="" width="500" /></p>
+</html>
+                  Figure 1.: 3D Model of H1F peptide Modelled by AlphaFold2.0 displayed in Pyomol
 <partinfo>BBa_K4165100 parameters</partinfo>
 <!-- -->