Difference between revisions of "Part:BBa K4165142"
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Tau binding peptide targeting the PHF seed of Tau | Tau binding peptide targeting the PHF seed of Tau | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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+ | MMD3rev peptide sequence is YWVSTHRAKLPD, it binds to 275-VQIINK-280 (PHF*) and 306-VQIVYK-311 (PHF) motifs of tau that are reported to drive tau paired helical filament aggregates. it inhibits formation of PHF6* and full‐length Tau aggregates, but not PHF driven aggregates, and prevents the formation of tau β‐sheet‐rich fibrils. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K4165142 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4165142 SequenceAndFeatures</partinfo> | ||
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+ | ===Dry Lab=== | ||
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+ | <p style=" font-weight: bold; font-size:14px;"> Modeling </p> | ||
+ | MMD3rev is modeled by AlphaFold2, Apptest, ITASSER and TrRosetta, best model obtained from ITASSER. | ||
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+ | <html> | ||
+ | <p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/mmd3rev.png" style="margin-left:200px;" alt="" width="300" /></p> | ||
+ | </html> | ||
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+ | Figure 1.: Predicted 3D structure of Synthetic peptide MMD3rev. | ||
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+ | <p style=" font-weight: bold; font-size:13px;"> Table 1: Quality assessment parameters of MMD3rev model. </p> | ||
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+ | <html> | ||
+ | <p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/mmd3rev-qa.png" style="margin-left:75px;" alt="" width="800" /></p> | ||
+ | </html> | ||
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+ | |||
+ | ===References=== | ||
+ | 1. Reference | ||
Revision as of 19:44, 5 October 2022
MMD3rev Peptide
Tau binding peptide targeting the PHF seed of Tau
Usage and Biology
MMD3rev peptide sequence is YWVSTHRAKLPD, it binds to 275-VQIINK-280 (PHF*) and 306-VQIVYK-311 (PHF) motifs of tau that are reported to drive tau paired helical filament aggregates. it inhibits formation of PHF6* and full‐length Tau aggregates, but not PHF driven aggregates, and prevents the formation of tau β‐sheet‐rich fibrils.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Dry Lab
Modeling
MMD3rev is modeled by AlphaFold2, Apptest, ITASSER and TrRosetta, best model obtained from ITASSER.
Figure 1.: Predicted 3D structure of Synthetic peptide MMD3rev.
Table 1: Quality assessment parameters of MMD3rev model.
References
1. Reference