Difference between revisions of "Part:BBa K4165097"

 
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<partinfo>BBa_K4165097 short</partinfo>
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<partinfo>BBa_K4165096 short</partinfo>
  
 
part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein
 
part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein
  
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===Usage and Biology===
 
===Usage and Biology===
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Synthetic peptide acquired by Phage display, Binds specifically to PDZ domain of HTRA1 and HTRA3 Proteins with G of –8.2 and IC50 of 2.5 ± 0.4
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4165097 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K4165096 SequenceAndFeatures</partinfo>
  
  
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K4165097 parameters</partinfo>
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Got score of 5 out of 6 in our Quality assessment code after modeling by AlphaFold2.0
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cbeta_deviations = 0, clashscore = 129.77, molprobity = 4.76, ramachandran_favored = 25, ramachandran_outliers = 75, Qmean_4 = -6.45183, Qmean6 = -5.513969
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===Modeling===
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<html>
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/htra1-bp/h1c.jpg" alt="" width="500" /></p>
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</html>
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                  Figure 1.: 3D Model of H1C peptide Modelled by AlphaFold2.0 displayed in Pyomol
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<partinfo>BBa_K4165096 parameters</partinfo>
 
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Revision as of 14:06, 6 October 2022


HTRA Binding Peptide 2

part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein

Usage and Biology

Synthetic peptide acquired by Phage display, Binds specifically to PDZ domain of HTRA1 and HTRA3 Proteins with G of –8.2 and IC50 of 2.5 ± 0.4

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

Got score of 5 out of 6 in our Quality assessment code after modeling by AlphaFold2.0 cbeta_deviations = 0, clashscore = 129.77, molprobity = 4.76, ramachandran_favored = 25, ramachandran_outliers = 75, Qmean_4 = -6.45183, Qmean6 = -5.513969

Modeling

                 Figure 1.: 3D Model of H1C peptide Modelled by AlphaFold2.0 displayed in Pyomol