Difference between revisions of "Part:BBa K200001"
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[[Image:II09_dam.jpg|400px|centre|<i>Figure 1:</i> Action of Dam methylase (source: <cite>#Dam1</cite>)]] | [[Image:II09_dam.jpg|400px|centre|<i>Figure 1:</i> Action of Dam methylase (source: <cite>#Dam1</cite>)]] | ||
| − | + | <centre>Figure: Action of Dam methylase (source: <cite>#Dam1</cite></centre> | |
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===Sequence and features=== | ===Sequence and features=== | ||
Revision as of 13:16, 2 September 2009
Dam methylase -> Dam
The methylase encoded by the dam gene ([http://en.wikipedia.org/wiki/Dam_(methylase) Dam methylase]) transfers a methyl group from S-adenosylmethionine to the N6 position of the adenine residues in the sequence GATC. When methylation occurs in the recognition site of a particular group of restriction endonuclease including MboI, this protects the DNA from cleavage.
Usage and Biology
DAM methylase (DNA adenine methylase) adds a methyl group to the adenine of the sequence 5'-GATC-3'in newly synthesized DNA. Dam methylases are important for several biological functions:
- [http://en.wikipedia.org/wiki/DNA_replication DNA replication]
- [http://en.wikipedia.org/wiki/Mismatch_repair DNA mismatch repair]
- Regulation of protein expression.
In the E.ncapsulator project, Dam methylases are used to protect the DNA from being destroyed by restriction enzymes DpnII (BBa_K200009) and TaqI (BBa_K200010).
<centre>Figure: Action of Dam methylase (source: #Dam1</centre>
Sequence and features
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 306
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]