Difference between revisions of "Part:BBa K200012"
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===Requirements/Limitations===
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*Possible crosstalk with other lambda repressors
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K200012 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K200012 SequenceAndFeatures</partinfo>

Revision as of 11:24, 2 September 2009

lambda promoter (cIts responsive)

This is different from the common lambda promoter in that it is able to be repressed by the temperature sensitive cI protein (BBa_K200011).

This lambda promoter contains 3 operator sites, as opposed to only 2 operator sites on the registry lambda promoter (BBa_R0051)

It has been shown through lysis experiments pL1 that this lambda promoter can be stringently repressed by the cI protein at temperatures up to 30°C. At temperatures higher than 30°C, the cI repressor is progressively thermally denatured and no longer able to bind to the promoter operator regions. Therefore, promoter activity is induced.

The system of the lambda promoter- temperature sensitive cI protein provides an effective way to regulate gene expression using temperature.


Requirements/Limitations

  • Possible crosstalk with other lambda repressors


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



References

<biblio> pL1 pmid=10227165 </biblio>