Difference between revisions of "Part:BBa K4119024"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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<partinfo>BBa_K4119024 parameters</partinfo> | <partinfo>BBa_K4119024 parameters</partinfo> | ||
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+ | <head> | ||
+ | <meta charset="UTF-8"> | ||
+ | <meta http-equiv="X-UA-Compatible" content="IE=edge"> | ||
+ | <meta name="viewport" content="width=device-width, initial-scale=1.0"> | ||
+ | <title>Document</title> | ||
+ | </head> | ||
+ | <body> | ||
+ | <p style="font-size: 180%; font-weight: bold;">Results:</p> | ||
+ | <p>The inhibition effect of the improved promoter(P2vgb) was stronger than that of the control group(Pvgb) under aerobic conditions. Under the induction of microaerobic conditions, the improved promoter(P2vgb) behaves similarly to the control group(Pvgb) under aerobic conditions. This suggests that tandem promoters improve protein expression.</p> | ||
+ | <p><img src="https://static.igem.wiki/teams/4119/wiki/jt-files/h2/img10.png" width="80%" height="80%"></p> | ||
+ | </body> | ||
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+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K4119024 SequenceAndFeatures</partinfo> |
Revision as of 11:04, 12 October 2022
Pvgb-double promoter->Bs2->Cpa fdx terminator
it's Pvgb-double promoter->Bs2->Cpa fdx terminator
Results:
The inhibition effect of the improved promoter(P2vgb) was stronger than that of the control group(Pvgb) under aerobic conditions. Under the induction of microaerobic conditions, the improved promoter(P2vgb) behaves similarly to the control group(Pvgb) under aerobic conditions. This suggests that tandem promoters improve protein expression.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 485
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 485
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 485
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 485
- 1000COMPATIBLE WITH RFC[1000]