Difference between revisions of "Part:BBa K4268010:Experience"

Revision as of 18:13, 8 October 2022

Level 0 parts BBa_R0085, BBa_B0034, BBa_K4268002 (Capsid Protein), and BBa_B0015 were cloned into the level 1 Golden Gate vector, PSB1K02. The ligation was then transformed into DH5α cells. After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.

Figure 1: Colony PCR of five colonies (clones) suspected of coating the insert Capsid Protein insert. The insert length is 720bp and the predicted size of the PCR product when using VF/VR primers is 1025 bp.

Applications of BBa_K4268010

User Reviews

UNIQ15281f65d6d0270c-partinfo-00000000-QINU UNIQ15281f65d6d0270c-partinfo-00000001-QINU

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 __NOTOC__
-This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
-how you used this part and how it worked out.
+Level 0 parts BBa_R0085, BBa_B0034, BBa_K4268002 (Capsid Protein), and BBa_B0015 were cloned into the level 1 Golden Gate vector, PSB1K02. The ligation was then transformed into DH5α cells. After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.
+[[File: T-suny-oneonta-t7-Level 1 capsid protein colony PCR figure.png|500px|thumb|center|Figure 1: Colony PCR of five colonies (clones) suspected of coating the insert Capsid Protein insert. The insert length is 720bp and the predicted size of the PCR product when using VF/VR primers is 1025 bp.]]
 ===Applications of BBa_K4268010===