Difference between revisions of "Part:BBa K200010"
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<partinfo>BBa_K200010 short</partinfo>
 
<partinfo>BBa_K200010 short</partinfo>
  
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This sequence codes for the restriction enzyme TaqI. This enzyme is Dam methylation sensitive.  
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This sequence codes for the restriction enzyme TaqI methyltransferase. TaqI Methyltransferase modifies the adenine residue (N6) of the sequence TCGA and is dam methylation sensitive.
  
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The recognition sequence is TCGA. TaqI is a 4 base cutting enzyme, leaving a 2 base overhang.
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TaqI is a 4 base cutter, leaving a 2 base overhang.
  
 
The cutting site is as shown:
 
The cutting site is as shown:
  
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TCGA  ->  T CGA
+
TCGA  ->  T / CGA
  
−
AGCT  ->  AGC T  
+
AGCT  ->  AGC / T  
  
  

Revision as of 12:48, 1 September 2009

TaqI Composite

This sequence codes for the restriction enzyme TaqI methyltransferase. TaqI Methyltransferase modifies the adenine residue (N6) of the sequence TCGA and is dam methylation sensitive.

TaqI is a 4 base cutter, leaving a 2 base overhang.

The cutting site is as shown:

TCGA -> T / CGA

AGCT -> AGC / T


As part of the Imperial 2009 iGEM E.ncapsulator project, TaqI was used along with DpnII to cut up the genetic material within the cell, completing the cell death module.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 36
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 175


References

[http://www.neb.com/nebecomm/products/productM0219.asp Information from NEB]