Difference between revisions of "Part:BBa K200008"
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<partinfo>BBa_K200008 short</partinfo>
 
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[http://en.wikipedia.org/wiki/Phenylalanine_hydroxylase Phenylalanine hydroxylase (PAH)] is the enzyme that determines the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder [http://en.wikipedia.org/wiki/Phenylketonuria phenylketonuria].<cite>#PAH1</cite>
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[http://en.wikipedia.org/wiki/Phenylalanine_hydroxylase Phenylalanine hydroxylase (PAH)] is the enzyme that breaks down [http://en.wikipedia.org/wiki/Phenylalanine phenylalanine] to [http://en.wikipedia.org/wiki/Tyrosine tyrosine]. Deficiency of this enzyme activity results in the autosomal recessive disorder [http://en.wikipedia.org/wiki/Phenylketonuria phenylketonuria].<cite>#PAH1</cite>
 
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The action of PAH is as follows:
 
  
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[http://en.wikipedia.org/wiki/Phenylalanine Phenylalanine] is broken down to [http://en.wikipedia.org/wiki/Tyrosine tyrosine], which is an essential step in the production of [http://en.wikipedia.org/wiki/Catecholamine catecholamines].
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PAH works well at a PH of around 7.0, and is denatured at temperatures above 30 degrees celsius (in experiments with gram-negative bacteria)<cite>#PAH2</cite>
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PAH works well at a PH of around 7.4, and is denatured at temperatures above 30 degrees celsius (in experiments with gram-negative bacteria)<cite>#PAH2</cite>
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Revision as of 10:54, 1 September 2009

Phenylalanine hydroxylase

[http://en.wikipedia.org/wiki/Phenylalanine_hydroxylase Phenylalanine hydroxylase (PAH)] is the enzyme that breaks down [http://en.wikipedia.org/wiki/Phenylalanine phenylalanine] to [http://en.wikipedia.org/wiki/Tyrosine tyrosine]. Deficiency of this enzyme activity results in the autosomal recessive disorder [http://en.wikipedia.org/wiki/Phenylketonuria phenylketonuria].#PAH1

PAH works well at a PH of around 7.0, and is denatured at temperatures above 30 degrees celsius (in experiments with gram-negative bacteria)#PAH2

Furthermore, the presence of oxygen and an additional cofactor (TPNH) is required for the hydrolysis to take place #PAH3.

Note that this part only works on gram-negative bacteria.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 287
    Illegal BamHI site found at 814
    Illegal XhoI site found at 524
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



References

[http://www.ncbi.nlm.nih.gov/nuccore/NM_000277.1?report=fasta Sequence from NCBI] <biblio>#PAH1 pmid=2014036 </biblio> <biblio>#PAH2 pmid=15708798 </biblio> <biblio>#PAH3 pmid=13525410 </biblio>