Difference between revisions of "Part:BBa K200003"
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<partinfo>BBa_K200003 short</partinfo>
 
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[[Image:http://upload.wikimedia.org/wikipedia/commons/a/a7/LPS.jpg | Thumb | left]]
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Waal ligase is an enzyme responsible for the ligation of an O-antigen to the core oligosaccharide in the Gram-negative bacterium's outer membrane.
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Unlike other exopolysaccharides, colanic acid does not naturally bind to the cell surface but rather forms a thick mesh between cells. While Waal Ligase usually links the O-antigen to the lipid A core, in K-12 it links colanic acid to the lipid A core. This is because E.coli K-12 does not produce O-antigen due to an insertion mutation.
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Unlike other exopolysaccharides, colanic acid does not naturally bind to the cell surface but rather forms a thick mesh between cells. While Waal Ligase usually links the O-antigen to the core oligosaccharide, in K-12 it links colanic acid to the core oligosaccharide. This is because E.coli K-12 does not produce O-antigen due to an insertion mutation.
  
 
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===Usage and Biology===
 
===Usage and Biology===
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Waal ligase is an enzyme responsible for the ligation of an O-antigen
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[[Image:II09_LPS.jpg | Thumb | left | <i>Figure 1:</i> LPS structure (source: [http://en.wikipedia.org/wiki/O_antigen wikipedia]]]
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LipoPolySaccharyde is a major component of the outer cell wall in Gram-negative bacteria. It is constituted of three main components: the O-antigen, the core polysaccharides and the lipid A (see Fig. 1).
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The O-antigen is a highly variable chain of polysaccharydes and is physically linked to the core.
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The core component is made up of a short chain of sugars (oligosaccharide) and is linked to both the O-antigen and the Lipid A components of the cell wall.
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The Lipid A component of the LPS contains fatty acid chains which causes the LPS to dock into the outer membrane of the Gram-negative bacterium.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K200003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K200003 SequenceAndFeatures</partinfo>
  
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===Requirements===
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*Gram-negative bacterium
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*Colanic acid producing gene ([[BBa_K200000 rcsB]]
  
 
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Revision as of 09:44, 1 September 2009

Rfal Waal ligase is an enzyme responsible for the ligation of an O-antigen to the core oligosaccharide in the Gram-negative bacterium's outer membrane.

Unlike other exopolysaccharides, colanic acid does not naturally bind to the cell surface but rather forms a thick mesh between cells. While Waal Ligase usually links the O-antigen to the core oligosaccharide, in K-12 it links colanic acid to the core oligosaccharide. This is because E.coli K-12 does not produce O-antigen due to an insertion mutation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 754
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Requirements


References

<biblio>

  1. function pmid=15838026
  2. Function2 pmid=19019161

</biblio>