Difference between revisions of "Part:BBa K200007"

Revision as of 08:42, 1 September 2009

Cellulase

This is a BioBrick for cellulase production in E.coli. CelE is one of the three major proteins of the cellulosome of Clostridium cellulolyticum.12

Cellulase catalyses the reactions, from crystal to cellulose to cellobiose/cellotetrose and to glucose. The cellulose is protease resistant.

As part of the Imperial 2009 iGEM E.ncapsulator project, E.coli was engineered to synthesise cellulase to a tunable threshold, and successfully coat and protect it through the stomach, until release in the small intestine.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 199
Illegal PstI site found at 700
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 700
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 287
Illegal BamHI site found at 814
Illegal XhoI site found at 524
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 199
Illegal PstI site found at 700
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 199
Illegal PstI site found at 700
1000
COMPATIBLE WITH RFC[1000]

References

[http://www.ncbi.nlm.nih.gov/nuccore/125972525?report=genbank&log$=seqview&from=961597&to=964041 Sequence from NCBI] <biblio>

1 pmid=9055408
2 pmid=10714996

</biblio>

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K200007 short</partinfo>
-This is a BioBrick for cellulase production in E.coli. CelE is one of the three major proteins of the cellulosome of Clostridium cellulolyticum.<cite>1</cite>
+This is a BioBrick for cellulase production in E.coli. CelE is one of the three major proteins of the cellulosome of Clostridium cellulolyticum.<cite>1</cite><cite>2</cite>
 Cellulase catalyses the reactions, from crystal to cellulose to cellobiose/cellotetrose and to glucose. The cellulose is protease resistant.
@@ Line 30: / Line 30: @@
 <biblio>
 #1 pmid=9055408
+#2 pmid=10714996
 </biblio>