Difference between revisions of "Part:BBa K200011"
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However, when the temperature is raised, typically to 42°C, the functionality of the protein is lost and the cI repressor is no longer able to bind to the operators on its promoter. Therefore, lambda promoter expression increases. <br> | However, when the temperature is raised, typically to 42°C, the functionality of the protein is lost and the cI repressor is no longer able to bind to the operators on its promoter. Therefore, lambda promoter expression increases. <br> | ||
| + | |||
| + | |||
| + | ===References=== | ||
| + | <biblio> | ||
| + | cI1 pmid=5274442 | ||
| + | cI2 pmid=10235623 | ||
| + | </biblio> | ||
Revision as of 19:56, 31 August 2009
The temperature sensitive lamda cI repressor protein has a cI857 mutation that results in denaturation of the repressor when the temperature is raised from 30 to 42°C, thereby allowing lambda promoter expression. cI1
The repressor normally negatively regulates the expression of genes from the bacteriophage lambda pL and pR promoters. This repressive action is strongest at 30°C. cI2
However, when the temperature is raised, typically to 42°C, the functionality of the protein is lost and the cI repressor is no longer able to bind to the operators on its promoter. Therefore, lambda promoter expression increases.
References
<biblio> cI1 pmid=5274442 cI2 pmid=10235623 </biblio>