Difference between revisions of "Part:BBa K4212049:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | PsspB is a promoter, which can aid the expression of gerA. GerA is one of the germinant receptors of B.subtilis, and it can respond to the presence of L-alanine and increase the germinantion rate of B.subtilis. The previous iGEM team iGEM17_SZU-China proved the superior transcription efficiency of this promoter. | |
Line 13: | Line 13: | ||
===Source=== | ===Source=== | ||
− | + | Synthetic construct. | |
===References=== | ===References=== |
Revision as of 13:56, 4 October 2022
Ger1
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3591
Illegal PstI site found at 606
Illegal PstI site found at 3542 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3591
Illegal PstI site found at 606
Illegal PstI site found at 3542 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3591
Illegal BglII site found at 3123
Illegal XhoI site found at 713 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3591
Illegal PstI site found at 606
Illegal PstI site found at 3542 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3591
Illegal PstI site found at 606
Illegal PstI site found at 3542
Illegal NgoMIV site found at 66 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 2778
Design Notes
PsspB is a promoter, which can aid the expression of gerA. GerA is one of the germinant receptors of B.subtilis, and it can respond to the presence of L-alanine and increase the germinantion rate of B.subtilis. The previous iGEM team iGEM17_SZU-China proved the superior transcription efficiency of this promoter.
Source
Synthetic construct.