Difference between revisions of "Part:BBa K4115023"

 
 
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The superfolder GFP with an LVA degradation tag is expressed by a constitutive promoter J23101 in this part.
 
The superfolder GFP with an LVA degradation tag is expressed by a constitutive promoter J23101 in this part.
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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It was used as one of the controls in starvation promoter characterization experiments.
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<br>
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Usually, the higher the external carbon source or nitrogen source, the higher the expression level.<br>
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The introduction of a degradation tag can help to eliminate the accumulation of expression over time, reduce the excessively bright fluorescence, and also make the sensitivity of starvation promoters better reflected.<br>
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4115023 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4115023 SequenceAndFeatures</partinfo>

Latest revision as of 13:58, 3 October 2022


J23101-B0034-sfGFP(with LVA)-B0015

The superfolder GFP with an LVA degradation tag is expressed by a constitutive promoter J23101 in this part.

Usage and Biology

It was used as one of the controls in starvation promoter characterization experiments.
Usually, the higher the external carbon source or nitrogen source, the higher the expression level.
The introduction of a degradation tag can help to eliminate the accumulation of expression over time, reduce the excessively bright fluorescence, and also make the sensitivity of starvation promoters better reflected.
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]