Difference between revisions of "Part:BBa K200004:Design"

(→‎Design Notes)
 
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===Design Notes===
 
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The Dam methylase was obtained through PCR using BL21 as the genomic DNA template. Primers were designed to include overhangs coding for XbaI and SpeI recognition sites in order to allow the gene to be BioBricked according to the BioBrick Standard.
 
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The primers are as follows:
 
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*Forward PCR primer containing XbaI overhang (bold) for BioBricking:<br>
 
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<br>
 
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<b>GCTCTAG</b>ATGAAGAAAAATCGCGCTTTTTTG<br>
 
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<br>
 
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*Reverse PCR primer containing SpeI overhang (bold) for BioBricking:<br>
 
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<br>
 
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<b>GGACTAGTA</b>TTATTTTTTCGCGGGTGAAAC<br>
 
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===Source===
 
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This is one of three site-specific DNA methylases found in most laboratory strains of E. coli.
 
  
 
===References===
 
===References===

Latest revision as of 14:36, 29 September 2009

TaqI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 18
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 157


References