Difference between revisions of "Part:BBa K4117008"

 
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<partinfo>BBa_K4117008 short</partinfo>
 
<partinfo>BBa_K4117008 short</partinfo>
  
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how to make CYP2C9
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We design a plasmid centering on CYP2C9 gene, which plays a indispensable role in the hydroxylation and oxygenization of Δ9-THC. Also, we specially choose some components for a more efficient protein expression.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 18:40, 11 October 2022


CYP2C9

We design a plasmid centering on CYP2C9 gene, which plays a indispensable role in the hydroxylation and oxygenization of Δ9-THC. Also, we specially choose some components for a more efficient protein expression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4467
    Illegal XbaI site found at 693
    Illegal SpeI site found at 713
    Illegal PstI site found at 1214
    Illegal PstI site found at 3118
    Illegal PstI site found at 4348
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4467
    Illegal SpeI site found at 713
    Illegal PstI site found at 1214
    Illegal PstI site found at 3118
    Illegal PstI site found at 4348
    Illegal NotI site found at 3275
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4467
    Illegal BamHI site found at 2887
    Illegal BamHI site found at 4377
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4467
    Illegal XbaI site found at 693
    Illegal SpeI site found at 713
    Illegal PstI site found at 1214
    Illegal PstI site found at 3118
    Illegal PstI site found at 4348
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4467
    Illegal XbaI site found at 693
    Illegal SpeI site found at 713
    Illegal PstI site found at 1214
    Illegal PstI site found at 3118
    Illegal PstI site found at 4348
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 732