Difference between revisions of "Part:BBa K249017:Design"
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<partinfo>BBa_K249017 short</partinfo> | <partinfo>BBa_K249017 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | Standard | + | Standard Prefix and Suffix, although there is BioFusion (aka Silver Lab) scar sequence fusing the YFP gene to the C-terminal Arginine Tag |
| + | This part was designed as an inversion device for YFP fluorescence. Upon addition of Arabinose the fluorescence of the protein was expected to drop significantly. This was observed. | ||
| + | [[Image:Results fig 11.png]] [[Image:Results fig 21.png]] | ||
===Source=== | ===Source=== | ||
Revision as of 14:56, 31 October 2009
N-terminal YFP --> Tet inverter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Standard Prefix and Suffix, although there is BioFusion (aka Silver Lab) scar sequence fusing the YFP gene to the C-terminal Arginine Tag
This part was designed as an inversion device for YFP fluorescence. Upon addition of Arabinose the fluorescence of the protein was expected to drop significantly. This was observed.
File:Results fig 11.png File:Results fig 21.png
Source
BioBrick Registry and synthesized Arganine Tag