Difference between revisions of "Part:BBa K4365003"
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Improvement of BBa_J04450 part. In the original construct, mRFP1 expression was observed irrespective of IPTG induction. So we replaced the inducible LacI-regulated promoter (BBa_R0010) with a constitutive (BBa_J23100) for colony screening. | Improvement of BBa_J04450 part. In the original construct, mRFP1 expression was observed irrespective of IPTG induction. So we replaced the inducible LacI-regulated promoter (BBa_R0010) with a constitutive (BBa_J23100) for colony screening. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K4365003 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4365003 SequenceAndFeatures</partinfo> | ||
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+ | ===Usage and Biology=== | ||
+ | [[File:BBa_K4365003.jpeg|400px|left|thumb|caption]] | ||
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Revision as of 15:21, 11 October 2022
turboRFP Selection Device with constitutive promoter
Improvement of BBa_J04450 part. In the original construct, mRFP1 expression was observed irrespective of IPTG induction. So we replaced the inducible LacI-regulated promoter (BBa_R0010) with a constitutive (BBa_J23100) for colony screening.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 683
Usage and Biology