Difference between revisions of "Part:BBa K249014:Design"
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<partinfo>BBa_K249014 short</partinfo> | <partinfo>BBa_K249014 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | Standard | + | Standard Prefix and Suffix, although there is BioFusion (aka Silver Lab) scar sequence fusing the YFP gene to the C-terminal Arginine Tag |
| + | This part was designed as an inversion device for YFP fluorescence. Upon addition of Arabinose the fluorescence of the protein was expected to drop significantly. This was observed. | ||
| + | [[Image:YFP fluorescence.jpg]] [[Image:YFP denaturing.jpg]] | ||
===Source=== | ===Source=== | ||
Revision as of 14:44, 31 October 2009
C-terminal YFP --> Tet inverter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Standard Prefix and Suffix, although there is BioFusion (aka Silver Lab) scar sequence fusing the YFP gene to the C-terminal Arginine Tag
This part was designed as an inversion device for YFP fluorescence. Upon addition of Arabinose the fluorescence of the protein was expected to drop significantly. This was observed.
Source
BioBrick Registry and synthesized Arganine Tag