Difference between revisions of "Part:BBa K4229064"

Revision as of 06:08, 12 October 2022

mTurquoise2 with C-Terminal SnoopCatcher

mTurqouise2 is with mVenus2(BBa_K4229062) one of our reporter genes, with which we tested the building and catching of the minimal(BBa_K4229047) and full wiffleball(BBa_K4229049) and SPD5 (BBa_K4229078). It has the snoopCatcher fused on the N-terminal of the protein to make it recruitable inside those compartments.

Principle of catching proteins via Spy/Snp-Catcher by T1 and their incorporation into the BMCs, illustrated as an example of incorporating mVenus in the full wiffleball

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 763
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 763
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 763
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 763
Illegal AgeI site found at 28
Illegal AgeI site found at 924
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4229064 short</partinfo>
-mTurquoise2 fused with snoopCatcher C-terminal
+mTurqouise2 is with mVenus2(BBa_K4229062) one of our reporter genes, with which we tested the building and catching of the minimal(BBa_K4229047) and full wiffleball(BBa_K4229049) and SPD5 (BBa_K4229078). It has the snoopCatcher fused on the N-terminal of the protein to make it recruitable inside those compartments.
+[[File:ReporterCatched.jpg|800px|thumb|left| Principle of catching proteins via Spy/Snp-Catcher by T1 and their incorporation into the BMCs, illustrated as an example of incorporating mVenus in the full wiffleball]]
 <!-- Add more about the biology of this part here