Difference between revisions of "Part:BBa K200008"
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Phenylalanine hydroxylase (PAH) encodes the enzyme phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria. | Phenylalanine hydroxylase (PAH) encodes the enzyme phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria. | ||
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As part of the Imperial 2009 iGEM E.ncapsulator project, E.coli was engineered to synthesise PAH to a tunable threshold, and successfully coat and protect it through the stomach, until release in the small intestine. | As part of the Imperial 2009 iGEM E.ncapsulator project, E.coli was engineered to synthesise PAH to a tunable threshold, and successfully coat and protect it through the stomach, until release in the small intestine. | ||
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Revision as of 21:06, 26 August 2009
Phenylalanine hydroxylase
Phenylalanine hydroxylase (PAH) encodes the enzyme phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.
As part of the Imperial 2009 iGEM E.ncapsulator project, E.coli was engineered to synthesise PAH to a tunable threshold, and successfully coat and protect it through the stomach, until release in the small intestine.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 287
Illegal BamHI site found at 814
Illegal XhoI site found at 524 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]