Difference between revisions of "Part:BBa K4220008"
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==Introduction== | ==Introduction== | ||
− | The product coded by this sequence is used to show the position of P2Y2 and the function of P2A by mCherry reporter. | + | *The product coded by this sequence is used to show the position of P2Y2 and the function of P2A by mCherry reporter. |
− | === | + | ===P2Y2=== |
− | In order to increase its sensitivity and maximum response to sense eATP (extracellular ATP), we mutated K240N and F307S.[1] | + | *In order to increase its sensitivity and maximum response to sense eATP (extracellular ATP), we mutated K240N and F307S.[1] |
− | === | + | ===P2A=== |
− | After adding two proteins in the two sides of P2A, it will cleave by itself and release the two proteins at the same time. | + | *After adding two proteins in the two sides of P2A, it will cleave by itself and release the two proteins at the same time. |
− | === | + | ===mCherry=== |
To report the successful cleavage of P2A peptide. | To report the successful cleavage of P2A peptide. |
Revision as of 01:35, 30 September 2022
P2Y2-P2A-mCherry
Introduction
- The product coded by this sequence is used to show the position of P2Y2 and the function of P2A by mCherry reporter.
P2Y2
- In order to increase its sensitivity and maximum response to sense eATP (extracellular ATP), we mutated K240N and F307S.[1]
P2A
- After adding two proteins in the two sides of P2A, it will cleave by itself and release the two proteins at the same time.
mCherry
To report the successful cleavage of P2A peptide.
Results
1.The P2A peptide can work effectively:
2.The production of P2Y2:
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1159
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1095
Illegal AgeI site found at 389 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 570
Illegal SapI site found at 615