Difference between revisions of "Part:BBa K4417000"
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<partinfo>BBa_K4417000 short</partinfo>
 
<partinfo>BBa_K4417000 short</partinfo>
  
 +
<h1>Description</h1>
 
Shuttle vecor for Bacillus subtilis. Mutated pCT5-bac2.0 by removing three BsaI sites to make it TypeIIS compatible.
 
Shuttle vecor for Bacillus subtilis. Mutated pCT5-bac2.0 by removing three BsaI sites to make it TypeIIS compatible.
  
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<span class='h3bb'>Sequence and Features</span>
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<h1>Sequence and Features</h1>
 
<partinfo>BBa_K4417000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4417000 SequenceAndFeatures</partinfo>
  

Revision as of 23:25, 6 October 2022


pCT5c Type IIS Compatible Plasmid

Description

Shuttle vecor for Bacillus subtilis. Mutated pCT5-bac2.0 by removing three BsaI sites to make it TypeIIS compatible.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3926
    Illegal EcoRI site found at 6982
    Illegal XbaI site found at 7437
    Illegal SpeI site found at 1
    Illegal PstI site found at 3174
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3926
    Illegal EcoRI site found at 6982
    Illegal NheI site found at 7213
    Illegal SpeI site found at 1
    Illegal PstI site found at 3174
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3926
    Illegal EcoRI site found at 6982
    Illegal BamHI site found at 907
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3926
    Illegal EcoRI site found at 6982
    Illegal XbaI site found at 7437
    Illegal SpeI site found at 1
    Illegal PstI site found at 3174
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3926
    Illegal EcoRI site found at 6982
    Illegal XbaI site found at 7437
    Illegal SpeI site found at 1
    Illegal PstI site found at 3174
    Illegal NgoMIV site found at 1937
  • 1000
    COMPATIBLE WITH RFC[1000]