Difference between revisions of "Part:BBa K4447004"

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FRET-based sensor system for the detection of erythromycin that consists of erythromycin C-12 hydroxylase [https://parts.igem.org/Part:BBa_K4447001 (BBa_K4447001)],an enzyme that catalyzes the oxidation of erythromycin, flanked by two fluorescent proteins: ECFP [https://parts.igem.org/Part:BBa_K1159302 (BBa_K1159302)]as an energy donor and Venus [https://parts.igem.org/Part:BBa_K1907000 (BBa_K1907000)]as an energy acceptor.
 
FRET-based sensor system for the detection of erythromycin that consists of erythromycin C-12 hydroxylase [https://parts.igem.org/Part:BBa_K4447001 (BBa_K4447001)],an enzyme that catalyzes the oxidation of erythromycin, flanked by two fluorescent proteins: ECFP [https://parts.igem.org/Part:BBa_K1159302 (BBa_K1159302)]as an energy donor and Venus [https://parts.igem.org/Part:BBa_K1907000 (BBa_K1907000)]as an energy acceptor.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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=Selecting Fluorescent Proteins=
 
=Selecting Fluorescent Proteins=
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<div style="text-align:justify;">

Revision as of 19:32, 29 September 2022


FRET-based system for the detection of erythromycin

FRET-based sensor system for the detection of erythromycin that consists of erythromycin C-12 hydroxylase (BBa_K4447001),an enzyme that catalyzes the oxidation of erythromycin, flanked by two fluorescent proteins: ECFP (BBa_K1159302)as an energy donor and Venus (BBa_K1907000)as an energy acceptor.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1913
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2562


Selecting Fluorescent Proteins