Difference between revisions of "Part:BBa K4447004"
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FRET-based sensor system for the detection of erythromycin that consists of erythromycin C-12 hydroxylase [https://parts.igem.org/Part:BBa_K4447001 (BBa_K4447001)],an enzyme that catalyzes the oxidation of erythromycin, flanked by two fluorescent proteins: ECFP [https://parts.igem.org/Part:BBa_K1159302 (BBa_K1159302)]as an energy donor and Venus [https://parts.igem.org/Part:BBa_K1907000 (BBa_K1907000)]as an energy acceptor. | FRET-based sensor system for the detection of erythromycin that consists of erythromycin C-12 hydroxylase [https://parts.igem.org/Part:BBa_K4447001 (BBa_K4447001)],an enzyme that catalyzes the oxidation of erythromycin, flanked by two fluorescent proteins: ECFP [https://parts.igem.org/Part:BBa_K1159302 (BBa_K1159302)]as an energy donor and Venus [https://parts.igem.org/Part:BBa_K1907000 (BBa_K1907000)]as an energy acceptor. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K4447004 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4447004 SequenceAndFeatures</partinfo> | ||
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| − | + | =Selecting Fluorescent Proteins= | |
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Revision as of 19:32, 29 September 2022
FRET-based system for the detection of erythromycin
FRET-based sensor system for the detection of erythromycin that consists of erythromycin C-12 hydroxylase (BBa_K4447001),an enzyme that catalyzes the oxidation of erythromycin, flanked by two fluorescent proteins: ECFP (BBa_K1159302)as an energy donor and Venus (BBa_K1907000)as an energy acceptor.
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Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1913
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2562