Difference between revisions of "Part:BBa K4447004"

Revision as of 19:08, 29 September 2022

FRET-based system for the detection of erythromycin

FRET-based sensor system for the detection of erythromycin that consists of erythromycin C-12 hydroxylase[1],an enzyme that catalyzes the oxidation of erythromycin, flanked by two fluorescent proteins: ECFP(BBa_K1159302)as an energy donor and Venus(BBa_1907000)as an energy acceptor.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1913
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2562

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 <partinfo>BBa_K4447004 short</partinfo>
-FRET-based sensor system for the detection of erythromycin that consists of erythromycin C-12 hydroxylase [https://parts.igem.org/Part:BBa_K4447001(BBa_K4447001)],an enzyme that catalyzes the oxidation of erythromycin, flanked by two fluorescent proteins: ECFP [https://parts.igem.org/Part:BBa_K1159302(BBa_K1159302)]as an energy donor and Venus [https://parts.igem.org/Part:BBa_1907000(BBa_1907000)]as an energy acceptor.
+FRET-based sensor system for the detection of erythromycin that consists of erythromycin C-12 hydroxylase[https://parts.igem.org/Part:BBa_K4447001(BBa_K4447001)],an enzyme that catalyzes the oxidation of erythromycin, flanked by two fluorescent proteins: ECFP[https://parts.igem.org/Part:BBa_K1159302 (BBa_K1159302)]as an energy donor and Venus[https://parts.igem.org/Part:BBa_1907000 (BBa_1907000)]as an energy acceptor.
 <!-- Add more about the biology of this part here