Difference between revisions of "Part:BBa K4345004"

Revision as of 14:41, 29 September 2022

NarX mutant fused to mPapaya with a rigid linker

For more information on NarX, go to this part page: BBa_K4345000. The mutant was designed based on a study of Cavicchioli et al., (1995). The histidine on place 399 was replaced by glutamic acid. This allows the NarX mutants to dimerize but blocks it from phosphorylating NarL (the second messenger).

This composite part consists of BBa_K4345008 (NarX H399-E), BBa_K4345002 (Rigid linker) and BBa_K4345003 (mPapaya).

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 2153
Illegal PstI site found at 659
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 2153
Illegal PstI site found at 659
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 2153
Illegal XhoI site found at 260
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 2153
Illegal PstI site found at 659
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 2153
Illegal PstI site found at 659
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4345004 short</partinfo>
-For more information on NarX, go to this part page: BBa_K4345000. The mutant was designed based on a study of Cavicchioli et al., (1995).
+For more information on NarX, go to this part page: BBa_K4345000. The mutant was designed based on a study of Cavicchioli et al., (1995). The histidine on place 399 was replaced by glutamic acid. This allows the NarX mutants to dimerize but blocks it from phosphorylating NarL (the second messenger).
 This composite part consists of BBa_K4345008 (NarX H399-E), BBa_K4345002 (Rigid linker) and BBa_K4345003 (mPapaya).