Difference between revisions of "Part:BBa K4461000"

Revision as of 14:12, 29 September 2022

dusB-fis promoter

The dusB is a member of the flavin mononucleotide (FMN)-dependent enzyme family that catalyzes the biosynthesis of D17 dihydrouridine in t-RNA, which is helpful in maintaining conformational flexibility of RNA and also important in ensuring the recognition of tRNA by specific aminoacyl-tRNA synthetases and the fidelity of translation.

Fis is a factor for inversion stimulation, a small DNA-binding and bending protein whose main role appears to be the organization and maintenance of nucleoid structure through direct DNA binding and by modulating gyrase and topoisomerase I production. Also, when saturated cultures of E. coli are diluted in rich media, the amounts of Fis protein and mRNA rapidly increase from undetectable to very high levels during the early logarithmic growth phase. The levels then decrease during late logarithmic growth and again become undetectable during the stationary phase.

Therefore, we want to use the dusB-fis promoter to regulate the fluorescent protein expression since early log phase.

Sequence and Features