Difference between revisions of "Part:BBa K4242003"
Jiangzhelin (Talk | contribs) |
|||
| Line 5: | Line 5: | ||
This part sequence originated from Pseudomonas aeruginosa encodes FleN protein. FleN is an important synergistic protein working with FleQ protein, FleN alone does not bind to DNA but FleQ and FleN interact in a FleQ/FleN/DNA complex. FleN stimulates the bending of pelA promoter DNA when FleQ and ATP or ADP are present. The DNA bending stimulated by FleN occurs only when ATP is present and disappears when c-di-GMP is added. | This part sequence originated from Pseudomonas aeruginosa encodes FleN protein. FleN is an important synergistic protein working with FleQ protein, FleN alone does not bind to DNA but FleQ and FleN interact in a FleQ/FleN/DNA complex. FleN stimulates the bending of pelA promoter DNA when FleQ and ATP or ADP are present. The DNA bending stimulated by FleN occurs only when ATP is present and disappears when c-di-GMP is added. | ||
| − | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | FleN plays an important role in P.aeruginosa, which exhibits antagonistic activity against FleQ. FleN and c-di-GMP cooperate to inhibit FleQ activity and, by extension, flagella synthesis in P. aeruginosa. FleN is a significant synergistic protein to work with FleQ. What’s more, In the presence of ATP, FleN forms dimers inducing a bending of the pelA promoter by bridging the bound FleQ proteins. In this situation, we can use c-di-GMP to regulate DNA bending caused by FleN and FleQ cooperation to control flagella biosynthesis genes and exopolysaccharide genes. We can use this function to explore medicine research. | |
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
| Line 13: | Line 12: | ||
| − | + | ===Functional Parameters: CUG_China=== | |
| − | ===Functional Parameters=== | + | |
<partinfo>BBa_K4242003 parameters</partinfo> | <partinfo>BBa_K4242003 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
Revision as of 11:36, 29 September 2022
FleN gene
This part sequence originated from Pseudomonas aeruginosa encodes FleN protein. FleN is an important synergistic protein working with FleQ protein, FleN alone does not bind to DNA but FleQ and FleN interact in a FleQ/FleN/DNA complex. FleN stimulates the bending of pelA promoter DNA when FleQ and ATP or ADP are present. The DNA bending stimulated by FleN occurs only when ATP is present and disappears when c-di-GMP is added.
Usage and Biology
FleN plays an important role in P.aeruginosa, which exhibits antagonistic activity against FleQ. FleN and c-di-GMP cooperate to inhibit FleQ activity and, by extension, flagella synthesis in P. aeruginosa. FleN is a significant synergistic protein to work with FleQ. What’s more, In the presence of ATP, FleN forms dimers inducing a bending of the pelA promoter by bridging the bound FleQ proteins. In this situation, we can use c-di-GMP to regulate DNA bending caused by FleN and FleQ cooperation to control flagella biosynthesis genes and exopolysaccharide genes. We can use this function to explore medicine research. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 765
- 1000COMPATIBLE WITH RFC[1000]