Difference between revisions of "Part:BBa K4365002:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | Was amplified using | + | Was amplified using primers from the p426 <ref>Mumberg D, Müller R, Funk M. (1995) Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds, Gene;156(1):119-122. doi:10.1016/0378-1119(95)00037-7</ref> |
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===Source=== | ===Source=== |
Latest revision as of 14:59, 11 October 2022
2 micron Ori
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 360
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 360
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 360
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Was amplified using primers from the p426 [1]
Source
p426 Mumberg plasmid
References
- ↑ Mumberg D, Müller R, Funk M. (1995) Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds, Gene;156(1):119-122. doi:10.1016/0378-1119(95)00037-7