Difference between revisions of "Part:BBa K4385001:Design"

 
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===Design Notes===
 
===Design Notes===
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PcusC is amplified from E. coli genomic DNA. The promoter can receive the upstream signal from the CusSR two-component membrane associated sensor kinase system, and inducibly express the downstream target gene. The expression intensity of PcusC has a certain positive correlation with the received signal intensity. By adjusting the external copper ion concentration, the expression intensity of PcusC can be adjusted.
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Lpp is an Escherichia coli lipoprotein, which can bind to the N-terminal of OmpA protein to help OmpA better localize the bacterial membrane, thus forming a mixed surface display system, which is more conducive to the display of macromolecular passenger proteins after binding.
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===Source===
 
===Source===

Latest revision as of 09:58, 1 October 2022


Lipoprotein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Lpp is an Escherichia coli lipoprotein, which can bind to the N-terminal of OmpA protein to help OmpA better localize the bacterial membrane, thus forming a mixed surface display system, which is more conducive to the display of macromolecular passenger proteins after binding.

Source

E.coli BL21 genomic sequence

References