Difference between revisions of "Part:BBa K4140005"
(→Usage) |
Ahmed Wael (Talk | contribs) (→Literature Characterization) |
||
| Line 11: | Line 11: | ||
L7Ae that binds to RNA inhibits the targeted transcript's ability to be translated. As it specifically targets a sequence on the 5' end of the RNA termed the 2x-kturn. as a post transcriptional modifier we use it to repress the translation of cas12g protein in case of high levels of phenylalanine and TyrR that leads to inhibiting CRISPR system activity and preserving the expression of PAH. | L7Ae that binds to RNA inhibits the targeted transcript's ability to be translated. As it specifically targets a sequence on the 5' end of the RNA termed the 2x-kturn. as a post transcriptional modifier we use it to repress the translation of cas12g protein in case of high levels of phenylalanine and TyrR that leads to inhibiting CRISPR system activity and preserving the expression of PAH. | ||
| − | == | + | ==Characterization of Mutational Landscape== |
| − | + | ||
| − | + | ||
| − | + | ||
| + | [[File:L7ae.png|thumb|Right|Figure 1. (shows the mutational landscape of the L7Ae protein) ]] | ||
| + | <br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br> | ||
==References== | ==References== | ||
Revision as of 08:01, 3 October 2022
L7AE
Part Description
A protein called L7Ae that binds to RNA inhibits the targeted transcript's ability to be translated. Transcriptional repressors take longer to suppress the intended construct than L7Ae that regulate gene expression at the translational level. As it specifically targets a sequence on the 5' end of the RNA termed the 2x-kturn. L7Ae can be used to build more complicated genetic circuits that are regulated at both the translational and transcriptional levels
Usage
L7Ae that binds to RNA inhibits the targeted transcript's ability to be translated. As it specifically targets a sequence on the 5' end of the RNA termed the 2x-kturn. as a post transcriptional modifier we use it to repress the translation of cas12g protein in case of high levels of phenylalanine and TyrR that leads to inhibiting CRISPR system activity and preserving the expression of PAH.
Characterization of Mutational Landscape
References
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]