Difference between revisions of "Part:BBa K4475006"
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This is the full exon only (cDNA) coding sequence for the CTB3 enzyme in the fungus Cercospora beticola. The enzyme is the second in a multi step pathway for the production of cercosporin, a reactive oxygen species producing toxin in the presence of sun light. FSU's iGEM team is looking to synthesize CTB3 in yeast as a proof of concept for cercosporin biosynthesis in a non-native and scalable organism, with the goal of producing cercosporin to combat algal blooms. The coding sequence has been codon optimized for Saccharomyces cerevisiae and modified to remove some illegal iGEM assembly cut sites via silent mutations. A 6x histidine tag is embedded on the N-terminus for expression verification.
 
This is the full exon only (cDNA) coding sequence for the CTB3 enzyme in the fungus Cercospora beticola. The enzyme is the second in a multi step pathway for the production of cercosporin, a reactive oxygen species producing toxin in the presence of sun light. FSU's iGEM team is looking to synthesize CTB3 in yeast as a proof of concept for cercosporin biosynthesis in a non-native and scalable organism, with the goal of producing cercosporin to combat algal blooms. The coding sequence has been codon optimized for Saccharomyces cerevisiae and modified to remove some illegal iGEM assembly cut sites via silent mutations. A 6x histidine tag is embedded on the N-terminus for expression verification.
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Source: CB0940_00834 6-hydroxytryprostatin B O-methyltransferase [Cercospora beticola] https://www.ncbi.nlm.nih.gov/gene/35424646
  
 
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Revision as of 06:57, 29 September 2022


CTB3 Gene from Cercospora beticola

This is the full exon only (cDNA) coding sequence for the CTB3 enzyme in the fungus Cercospora beticola. The enzyme is the second in a multi step pathway for the production of cercosporin, a reactive oxygen species producing toxin in the presence of sun light. FSU's iGEM team is looking to synthesize CTB3 in yeast as a proof of concept for cercosporin biosynthesis in a non-native and scalable organism, with the goal of producing cercosporin to combat algal blooms. The coding sequence has been codon optimized for Saccharomyces cerevisiae and modified to remove some illegal iGEM assembly cut sites via silent mutations. A 6x histidine tag is embedded on the N-terminus for expression verification.

Source: CB0940_00834 6-hydroxytryprostatin B O-methyltransferase [Cercospora beticola] https://www.ncbi.nlm.nih.gov/gene/35424646

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1263
    Illegal XbaI site found at 100
    Illegal XbaI site found at 457
    Illegal XbaI site found at 925
    Illegal XbaI site found at 1243
    Illegal XbaI site found at 1381
    Illegal XbaI site found at 1651
    Illegal XbaI site found at 2104
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1263
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1263
    Illegal BglII site found at 388
    Illegal BglII site found at 562
    Illegal BglII site found at 1291
    Illegal BglII site found at 2563
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1263
    Illegal XbaI site found at 100
    Illegal XbaI site found at 457
    Illegal XbaI site found at 925
    Illegal XbaI site found at 1243
    Illegal XbaI site found at 1381
    Illegal XbaI site found at 1651
    Illegal XbaI site found at 2104
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1263
    Illegal XbaI site found at 100
    Illegal XbaI site found at 457
    Illegal XbaI site found at 925
    Illegal XbaI site found at 1243
    Illegal XbaI site found at 1381
    Illegal XbaI site found at 1651
    Illegal XbaI site found at 2104
  • 1000
    COMPATIBLE WITH RFC[1000]