Difference between revisions of "Part:BBa K4475000"
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This is the full exon only (cDNA) coding sequence for the CTB1 enzyme in the fungus cercospora beticola. The enzyme is the first in a multi step pathway for the production of cercosporin, a reactive oxygen species producing toxin in the presence of sun light. FSU's iGEM team is looking to synthesize CTB1 in yeast as a proof of concept for cercosporin biosynthesis in a non-native and scalable organism, with the goal of producing cercosporin to combat algal blooms. The coding sequence has been codon optimized for Saccharomyces cerevisiae and modified to remove some illegal iGEM assembly cut sites via silent mutations. An HA tag is embedded on the N-terminus for expression verification.
 
This is the full exon only (cDNA) coding sequence for the CTB1 enzyme in the fungus cercospora beticola. The enzyme is the first in a multi step pathway for the production of cercosporin, a reactive oxygen species producing toxin in the presence of sun light. FSU's iGEM team is looking to synthesize CTB1 in yeast as a proof of concept for cercosporin biosynthesis in a non-native and scalable organism, with the goal of producing cercosporin to combat algal blooms. The coding sequence has been codon optimized for Saccharomyces cerevisiae and modified to remove some illegal iGEM assembly cut sites via silent mutations. An HA tag is embedded on the N-terminus for expression verification.
  
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Source
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Source:
 
CB0940_00833 CTB1 [Cercospora beticola]
 
CB0940_00833 CTB1 [Cercospora beticola]
 
https://www.ncbi.nlm.nih.gov/gene/35424645
 
https://www.ncbi.nlm.nih.gov/gene/35424645

Revision as of 06:54, 29 September 2022


CTB1 Gene from Cercospora beticola

This is the full exon only (cDNA) coding sequence for the CTB1 enzyme in the fungus cercospora beticola. The enzyme is the first in a multi step pathway for the production of cercosporin, a reactive oxygen species producing toxin in the presence of sun light. FSU's iGEM team is looking to synthesize CTB1 in yeast as a proof of concept for cercosporin biosynthesis in a non-native and scalable organism, with the goal of producing cercosporin to combat algal blooms. The coding sequence has been codon optimized for Saccharomyces cerevisiae and modified to remove some illegal iGEM assembly cut sites via silent mutations. An HA tag is embedded on the N-terminus for expression verification.

Source: CB0940_00833 CTB1 [Cercospora beticola] https://www.ncbi.nlm.nih.gov/gene/35424645

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3532
    Illegal BamHI site found at 3441
    Illegal BamHI site found at 5614
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6121
    Illegal AgeI site found at 4195
  • 1000
    COMPATIBLE WITH RFC[1000]