Difference between revisions of "Part:BBa K4193002:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
not yet
+
By using a RNA-based gene-regulatory platform, we design a ribozyme switch linked behind YopE mRNA, it contains a sensor domain, comprised of an aptamer sequence, and an actuator domain, comprised of hammerhead ribozyme sequence.
 +
<html>
 +
<head>
 +
<meta charset="utf-8">
 +
 +
</head>
 +
<body>
 +
<div>
 +
<img src="https://2020.igem.org/wiki/images/7/7e/T--OUC-China--result_fig2_th.jpg" style="width: 80%;">
 +
</div>
 +
</body>
 +
</html>
  
  

Revision as of 04:52, 8 October 2022


ribozyme riboswitch


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 38
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 38
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 38
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 38
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

By using a RNA-based gene-regulatory platform, we design a ribozyme switch linked behind YopE mRNA, it contains a sensor domain, comprised of an aptamer sequence, and an actuator domain, comprised of hammerhead ribozyme sequence.


Source

not yet

References